Continuous Microfluidic Immunosensing with Antibody Conjugated Paramagnetic Beads
نویسندگان
چکیده
A microdevice assay is being developed to continuously measure specific antigen concentrations within a sample stream. This device will allow real-time measurement of systemic protein concentrations within blood plasma, at high sample rates not possible with existing bench-top assays. Magnetic actuation of antibody coated paramagnetic microbeads between sample and wash streams leads to highly controlled residence times for accurate and reproducible measurements. A two-stage antigen sandwich assay is used to determine the antigen concentration based on fluorescence intensity of a secondary antibody. The microdevice can act as an on-chip Enzyme-Linked Immuno-Sorbent Assay (ELISA) with real time tracking of antigen concentration.
منابع مشابه
Comparison of Two Immunomagnetic Separation Methods for Detection of Mycobacterium avium subsp. Paratuberculosis in Bovine Feces
Detection of Mycobacterium paratuber culosis in feces is complicated by the slowdivision and the lipid composition of the cell wall of the bacterium. In this study, we compared a rapid and sensitive method for direct detection of M. paratuberculosis with anti-paratuberculosis chicken IgY-conjugated magnetic nanoparticles versus commercially available micro-sized paramagnetic beads. Tenfold seri...
متن کاملMicrofluidic capturing-dynamics of paramagnetic bead suspensions.
We study theoretically the capturing of paramagnetic beads by a magnetic field gradient in a microfluidic channel treating the beads as a continuum. Bead motion is affected by both fluidic and magnetic forces. The transfer of momentum from beads to the fluid creates an effective bead-bead interaction that greatly aids capturing. We demonstrate that for a given inlet flow speed a critical densit...
متن کاملMagnetic force-based multiplexed immunoassay using superparamagnetic nanoparticles in microfluidic channel.
This paper describes a novel microfluidic immunoassay utilizing binding of superparamagnetic nanoparticles to beads and deflection of these beads in a magnetic field as the signal for measuring the presence of analyte. The superparamagnetic 50 nm nanoparticles and fluorescent 1 microm polystyrene beads are immobilized with specific antibodies. When target analytes react with the polystyrene bea...
متن کاملElectrochemical magneto immunosensing of antibiotic residues in milk.
A novel electrochemical immunosensing strategy for the detection of sulfonamide antibiotics in milk based on magnetic beads is presented. Among the different strategies for immobilizing the class-specific anti-sulfonamide antibody to the magnetic beads--such as those based on the use of Protein A or carboxylate modified magnetic beads - ,the best strategy was found to be the covalent bonding on...
متن کاملMicrofluidic immunomagnetic multi-target sorting--a model for controlling deflection of paramagnetic beads.
We describe a microfluidic system that uses a magnetic field to sort paramagnetic beads by deflecting them in the direction normal to the flow. In the experiments we systematically study the dependence of the beads' deflection on bead size and susceptibility, magnet strength, fluid speed and viscosity, and device geometry. We also develop a design parameter that can aid in the design of microfl...
متن کامل